This laboratory manual has been specifically designed to integrate undergraduate students as fully as possible into the modern practices of cell biology. While not always feasible because of finances, availability, etc., undergraduates should be exposed to and actually allowed to use the equipment much in evidence in a modern cell biology research laboratory. Cell biology is a young and rapidly expanding science. A proper presentation of the field leaves educators little choice but to open up the door of the field leaves educators little choice but to open up the door of the research laboratory and to let in the undergraduate student. This handbook is not intended to cover the entire field of cell biology. A brief introduction precedes each exercise, but it is expected that a text book and/or lecturer will introduce the basic principles needed to understand the rationales of a particular project. The manual is designed to create a choice of projects for the student and instructor. For example, cell fractionation is often the first step in a project. Many options are possible for appropriate analysis. It is hoped that the instructor and students will discuss and explore experimental alternatives for each exercise.