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This laboratory manual has been specifically designed to integrate
undergraduate students as fully as possible into the modern practices of cell
biology. While not always feasible because of finances, availability, etc.,
undergraduates should be exposed to and actually allowed to use the equipment
much in evidence in a modern cell biology research laboratory. Cell biology is a
young and rapidly expanding science. A proper presentation of the field leaves
educators little choice but to open up the door of the field leaves educators
little choice but to open up the door of the research laboratory and to let in
the undergraduate student. This handbook is not intended to cover the entire
field of cell biology. A brief introduction precedes each exercise, but it is
expected that a text book and/or lecturer will introduce the basic principles
needed to understand the rationales of a particular project. The manual is
designed to create a choice of projects for the student and instructor. For
example, cell fractionation is often the first step in a project. Many options
are possible for appropriate analysis. It is hoped that the instructor and
students will discuss and explore experimental alternatives for each exercise.
Table of Contents :
# Introduction to Data Gathering and Analysis Using Simple Membrane Permeability
and Enzyme Kinetics Data as Experimental Models # Liposome Formation as a Model
for Cell Evolution # Observations and Exercises Concerning the Use and Care of
Light Microscopes # Light Microscope Observation of Cell Structure Using
Specific Staining Techniques # Growth Kinetics in Yeast Cell Cultures # pH and
Buffers # Spectrophtometry # Introduction to the Quantitative Assay of Enzymatic
Activity: Triose Phosphate Isomerase # The Kinetic Characterization of Alcohol
Dehydrogenase # Rat Liver Fractionation by Differential Centrifugation #
Analysis of Mitochondrial Succinate Dehydrogenase # Isolation of Castor Bean
Endosperm Organelles Using Density Gradient Centrifugation # Assay of Lecithin
Synthesis by Cholinephosphotransferase # Determination of Molecular Weight Using
# Separation of Proteins Using Gel Filtration Chromatography * Investigation of
Transcription in Isolated Rat Liver * Light-Driven Proton Pumping in Spinach *
Translation in Pea Chloroplasts * Restriction Endonuclease Analysis of Nuclear *
Immunocytochemical Analysis of the Cytoskeleton of Cultured Animal Cells
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